Abstract
It has been known that dopamine is very important in olfactory associated learning of drosophila. And in the recently research, it has been postulated that dopamine is only needed in the acquisition of punish learning. Therefore, dopaminergic neurons has been implicated to play a key role in delivering aversive unconditional stimulus (US) to the MBs to associate with olfactory conditional stimulus (CS) during classical associated learning in the drosophila. By using fluorescence microscopy, we found that the pattern of TH-GAL4, Cha-GAL80/ UAS-mCD8::GFP was different with TH-GAL4 / UAS-mCD8::GFP. In the TH-GAL4, Cha-GAL80/ UAS-mCD8::GFP line, dopamine neurons innervating in MB calyx were subtracted, and dopamine neurons innervating MB lobes were not . To find out where CS/US associates, we were performing behavior analysis by expressing Shits1 in TH-Gal4, Cha-Gal80 line and TH-Gal4 line. And we find learning of TH-GAL4, Cha-GAL80/ UAS- Shits1 was normal at restricted temperature while learning of TH-GAL4/ UAS- Shits1 was blocked. Besides dopamine, octopamine play a key role in olfactory associated learning of drosophila, too. But octopamine is only needed in appetitive learning. If we want to perform behavior analysis in octopamine neurons, we must perform a good protocol of reward leaning. Unfortunately, the protocol of reward learning is too rough to gain stable Performing index (PI). In this research we can only show how to get stable PI in wild type.