Abstract
Abstract Cancer is the most dangerous disease in the world, despite their have a lot of treatment, however cancer doesn’t disappear from human. In the traditional chemotherapeutic agents which used for treating cancer not only have no specificity on normal cell, but also it will have a lot of side effect, even the cancer cell will develop resistant ability. Hence, human need to discover novel anti-cancer drugs against different types of cancer. In the recent research have pointed out the therapeutic potential drugs, various bacteriocins. In this thesis we use BD21whcih bacteriocin belongs to class II and have anticancer ability as model to exchange its amino acids for pursuing more higher cytotoxicity to colon cancer. We found a protein that just need half concentration to kill colon cancer.