Abstract
Abstract Parkinson’s disease is a disabling movement disorder due to thedegeneration of substantia nigra pars compacta (SNc) dopamine neurons. DBS-STN (Deep Brain Stimulation Subthalamic Nucleus), which is thought to affect the pathological neuronal activity and abnormal oscillactions in the basal ganglia caused by the loss of dopamine in the nigrostriatal system, is now the most frequently practiced surgical therapy for treating PD patients. In the present study, we have successfully induced PD model rats for development an integrated electrophysiology instrument suitable for the DBS procedure. The major purpose of this study is to identify which brain regions would be activated after DBS-STN. In our studies, we used c-Fos immunostaining to label rat brain regions that were activated under different conditions. We found that the neuronal activity of subthalamic nucleus (STN), substantia nigra (SN), globus pallidus (GP), caudate putamen(CPu), motor cortex, habenular nucleus (Hb) and paraventricular thalamic nucleus posterior (PVP) were not affected by 130 Hz DBS, but the neuronal activity of these areas increased by 1300 Hz DBS in normal rats. The result indicated that the super high frequency would increase a wide range of neuronal activity of the brain. However, the PD rats’ neuronal activity of these areas was in hyperactive state before DBS. After 130 Hz DBS, the neuronal activity of CPu, GP, motor cortex would decrease but the neuronal activity increased in Hb and PVP. These results suggested that HFS-DBS would change the neuronal activity of GP, CPu, Motor cortex, Hb and PVP in the PD rats. It may be related to the alleviation of the amphetamine-induced rotation.