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改善GCRMA中移除生物晶片光學干擾的方法
Thesis

改善GCRMA中移除生物晶片光學干擾的方法

Ming-Jie Hsieh
Masters, 國立清華大學, 統計學研究所
2005

Abstract

GCRMA Optical Affymetrix
High density oligonucleotide microarrays have been one of the most useful techniques which widely used in genomic science. GCRMA is designed to measure the gene expression on the microarrays, which uses a very simple global estimator for the optical noise, and we suggest correcting the optical noise in another location-specific manner in this study. We found the base-effects estimated by location specific optical adjusted data are stronger than those by global optical adjusted data, and there is a little improvement in accuracy for several spike-in probe sets on the used Affymetrix array.

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