Abstract
The thesis presents acontrolled microfluidic system which consists of laminar stream-based microchannels. This characteristic is useful for selecting motile sperm.There is some laminar flow phenomenon in chip because flow rate of sperm and buffer are different. Motile sperm has ability to pass through the laminar flow boundary, and reach the reservoir we want to collect. The bio-chip method is different from traditional methods and centrifugation. Centrifugation need to select sperm by rotating at fast speed, the great gravity maybe will cause the damage to sperm. Finally, the flow cytometric analysis is utilizedto confirm the sorting efficiency of this system. The result showed that our study is successfully for sorting sperm. Assisted Reproductive Technology (ART) has become an important role in the field of medical science. It provides an important solution to some couple which is suffer from infertility.No matter ICSI (Intracytoplasmic sperm injection) or IVF (in vitro fertilization), sperm motility, quantity, concentration are the main reason for fertilization. Therefore, the optimized microfluidic system provides a perfect opportunity to use an inexpensive requirement to select the most appropriate sperms before IVF process. The fabrication process of bio-chip include SU8 thick-film photolithography and soft-lithography which is used by PDMS (Polydimethylsiloxanes), which has the advantages of biocompatibility, low-cost, high-precision, disposable, and easy to produce etc., so that it is suitable and common used for biomedical chip. The study is to construct a microfluidic based controlled microfluidic system, which focus on the sorting and the classification based on sperm motility and viability. The experimental details including chip design, flow field simulation, pump parameter setup, flow cytometric analysis. All parts are a key point associated with the final result.Slow flow rate has favorable environment to increase the opportunity of motile sperm to pass through the laminar flow boundary. Moreover, this environment can reduce interference with direction of sperm swim forward. At the last, more progressive quantity and quality can be observed by the flow cytometric analysis. The result show that counted ratio of motile sperm can increase a lot. Therefore, using controlled microfluidic system is successful to enhance the sperm motility sorting efficiency. It confirms that the microchannel really has an advantage in Assisted Reproductive Technology.