Abstract
Cancer results from the uncontrolled growth of abnormal cells in the body. The death rate caused due to cancer has been rising every year. Lung cancer among all cancers has the most significant impact on death of people affected by cancer. The diagnostics are committed to develop cure strategies. Although there are many remedies to treat different kind of cancer such as surgery, radiation and chemotherapy, but the success rate is still low. With the advance in medical approach, there are many new cancer treatments which have been developed in recent years. According to the literature survey and clinical experts’ comments, the ability of a tumor to achieve vascularization is an essential hallmark of cancer biology. Recently, cell-based therapy has been getting important for the inhibition of tumor angiogenesis. It can be a ray of hope to cure cancer for patients suffering from cancer. With the rapid development in MEMS technology, tissue reconstruction can be realized in vitro which can mimic the biological function in vivo and enable biologically meaningful data to be extracted during cell-based assays. For some patients, predicting the outcome of a treatment by performing in vitro cellular drug screening or trying to find the other suitable therapy is desirable, as it can avoid the problem of legal and ethical controversies. In this study, we try to integrate the microfluidic bio-chip, the manipulation of cells via dielectrophoresis patterning, bio-MEMS and photolithography techniques. The spatial orientation arrangement of cells via the cell patterning technique developed in our group was used to realize the reconstruction of tumor microenvironment. We can not only use this model to study the cell interaction of the cancer cell and the vascular endothelial cell but also define the mechanical stiffness of ECM that will influence the angiogenesis. At the same time, it can mimic the area of tumor angiogenesis where the cell migration phenomenon appears. It will provide pharmaceutical information and aid in drug discovery for cancer. The development in this research will provide pharmaceutical information and aid in drug discovery for cancer treatment.