Abstract
Digital microfluidic system is a potential technique for transporting droplet. Using the phenomenon of EWOD, we can control small droplet composing of drugs, particles and cells. Every droplet can be controlled individually to prevent contamination. In recent years, Lab-Chip technique uses the platform of digital microfluidic system extensively, and integrates various functions, such as cell sorting, cell culturing and droplet heating. In this paper, I report an integrated microfluidic system to demonstrate droplet transportation and cell patterning on the same chip. We have designed a fishbone-like electrode, based on the feature of EWOD phenomenon. With this novel design, single voltage signal was used to transport droplets for a distance of 4 cm without the need of complicated control circuits. In addition, it can transport droplet with a volume of 30μl to 370μl. We also design a circular array electrode to pattern lung cancer cells for mimicking the island-like morphology of the lung cancer tissue. Meanwhile, we already use MTT assay to observe the increased viability of (H287) lung cancer cells which was co-cultured with (3T3) fibroblast and treated with Iressa. I successfully build the model of man-made lung cancer tissue.