Abstract
Bacterial cell surface layer proteins (S-layers) were the outermost cell envelope component of many bacteria and archaea. Deinococcus radiodurans, had a complex cell wall profile that including S-layers. Traditionally, in preparations, S-layers of D. radiodurans were usually isolated by chemical reagents treatment; in applications, S-layers were usually used in two-dimension form. We wanted to build up a method to efficiently isolate S-layers from D. radiodurans and research the development of S-layers in three-dimension form. We found that we succeeded in building up an easy and novel method to isolate the S-layers fraction (SLF). Furthermore, we firstly used physical force to make SLF to form particles (SLFP), diameter ~100 – 200 nm. Secondary, when Ag+ was added to SLF buffer solution at neutral condition, we unexpectedly found there were hollow spheres called SLF-Ag, the diameter ~1 – 30 mm. These results indicated that the novel method could efficiently isolate S-layers from D. radiodurans. SLF could form three-dimension hollow spheres and then they were useful, plasticity, and still owning inherent properties. Moreover, the meaning of the SLF-Ag may be about the implication of biogenic minerals, polarity, and evolution.