Abstract
Though we sort out the cobra in nerve toxicity in the general idea, some red and swollen in local tissue and limbs necrosis are downright bad that emerges from Taiwan cobra(Naja atra) bite mainly, and the necrosis tissue is apt to infect with the Gram's negative bacterium, and the nerve toxicity: blocking neuron— muscle conducts and difficulty in breathing, just account for 5% . Study points out that the necrosis does not cause by bacterial infection, but by cardiotoxin of snake venom. We find that in the east and western Taiwan cobra venom, not merely its poisonous strength power is obviously different, and the composition of the cardiotoxin has different ways, we suspect that this may cause the wound injury in different degrees, so we has directed against the different toxin proteins in the east and western Taiwan cobra venom and make toxicity comparison. Because of the functional mechanism of the cardiotoxin is one kind of the membrane toxin, and the evidence shows that the cardiotoxin will cause the necrosis of the skeletal muscle cell, so we observe the cell toxicity and myotoxicity of the snake venom in the experiment . The result shows that the toxin proteins in the snake venom obviously have synergistic effect, and the east and western cobra venom really have different toxicity in physiology . Infer according to observation of the cell and animal experiment, by the same dosage of snake venom, the west(white belly 1) cobra have the highest cell toxicity and myotoxicity ; the cobra of east(black belly) and another type of west(white belly 2) have difference each other . And the differences are come from cardiotoxin obviously . To compare with different toxin proteins, we find that in the west( white belly 1) cobra, the most contents— cardiotoxin A3 has the strongest cell and muscle toxicity ; and in the west(white belly 2) cobra, the higher contents—cardiotoxin A2, A4 have stronger cell toxicity ; and higher contents of the east(black belly) cobra, cardiotoxin A1, A6 have stronger myotoxicity . If we consider the venom quantity by every single time which the snake bites, then the myotoxicity of the east(black belly) cobra should exceed more than 4 times of cobra in the west (white belly).