Abstract
It is important for revealing the mechanisms of wound healing. It was known that JNK signaling pathway component, puckered, expresses in the epithelium cells around the wound. In genetic research of wound healing process of Drosophila, pucE69 was usually used as a lacZ marker. To observe the dynamic motion of wound healing process of Drosophila, we constructed the GFP reporters by the upstream non-coding DNA sequence of puckered. The GFP reporter containing 1.9kb non-coding DNA sequence upstream of puckered (pucF43-pGP) was sufficient to express around the wound of adults and larva. By the measurement of GFP expression area, pucF43-pGP has larger area than Ddc-GFP after 10 hours. However, even the 3.7kb non-coding DNA sequence could not drive the GFP reporter (pucF63-pGP) to express in the leading edge cells during dorsal closure. It suggests that there are different mechanisms driving puckered expression during wound healing and dorsal closure. Additionally, we predicted some transcriptional factor binding sites in pucF43 sequence, which also exist in upstream of Ddc. It is probably that there was a factor driving both puckered and Ddc during wound healing process.