Abstract
The gustatory system elicits innate behavior critical for directing animals to ingest nutritious substrate and avoid toxic compound. However, the neural circuits map for gustatory system is still not clear. In this study, Firstly, we analysis the axonal terminal distribution of different GRNs by mapping the expression patterns of the first level of gustatory circuit- gustatory receptors neurons (GRNs) reported from previous studies. Secondly, we trace the candidate gustatory projection neuron by PA-GFP and fly circuit database. We also predict the higher brain center that mediates certain gustatory behavior from neuron morphology. Thirdly, we developed the feeding behavior assay to survey the gustatory circuits in the Drosophila brain. In our preliminary forward genetic screening, found some gene trap lines are identified as a gustatory mutant. Finally, we also map and identify the neurotransmitter release from the gustatory modulatory circuit- hugin and dilp2 neurons. In future, we will screen the gustatory circuit large scale and verify the candidates of gustatory circuits in the secondary level by feeding behavior assay.