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極低頻電磁場對細胞存活之研究
Thesis

極低頻電磁場對細胞存活之研究

陳昶瑞
Masters, National Tsing Hua University
1998

Abstract

極低頻電磁場基因晶片細胞存活分析Helmholtz 線圈 ELF electromagnetic fieldsmicroarraycell survivalHelmholtz coils
Ionization radiation can destroy the binding between molecules caused single strand break (SSB) or double strands break (DSB) through its high energies. Carcinogenesis, cell deaths have strong relationship with SSB, and DSB. In contrast, energies carried by Extremely Low Frequency electromagnetic fields are not sufficient to denature the bindings. In recent review of some related studies, ELF may increase the SSB or DSB. In general, when the SSB or DSB occurred, it will be repaired automatically. The triggering of repair mechanism was conducted by some weak transduction signals. In this study, our concerns will focus on these signals which may interfered by the ELF electromagnetic fields, and changed some repair mechanisms. For our approaches, exposing 2Gy and 4Gy-dosage ray on Nfsa cell to cause SSB or DSB, then exposed in 5 Gauss magnetic field for 1 to 4 hours. Afterwards we use the colorimetric analysis to estimate amount of cell and survival fractions for investigating whether repair mechanisms infected by ELF electromagnetic fields. As our results, we can not conclude the possibilities for our suggestions. Meanwhile we had built a microarray system, to study whether the gene expressions changed after the exposure by ELF electromagnetic field in future.

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