Abstract
In this thesis, we use the CFB (cell free broth) of Deinococcus radiodurans stationary phase culture as a system to fabricate silver nanowire. After series of experiments, we found that the amount of silver nanowires fabricated by D. radiodurans CFB (with 2.5 x 105 wires / mL, average length is about 62.5 ± 9.1 µm) are better than those fabricated by cell culture (with 3.5 x 103 wires / mL, average length is about 38.3 ± 2.3 µm). And the ability to fabricate silver nanowire is growth-phase dependent, emerging at late-log phase, and optimizing at stationary phase. Furthermore, the ability to fabricate silver nanowire could be induced by CFB ( 0.01％, v/v ). Besides that, we also found the CFB is heat-tolerance. But the reaction of silver nanowire synthesis is not heat stable, and must react under neutral pH. In conclusion, using CFB system to fabricate silver nanowire, could provide more silver nanowires for further research and development. And by studying the factors which influence the silver fabricated ability of CFB, could be a way to know more about cell-to-cell interaction of D. radiodurans.