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甲蟲蟑螂之咽側腺在胚胎發育後期的超顯微結構變化
Thesis

甲蟲蟑螂之咽側腺在胚胎發育後期的超顯微結構變化

李苑如
Masters, 國立清華大學, 生命科學系
1995

Abstract

青春激素, 胚胎發育後期, 活性 juvenile hormone, late embryonic stage, activity
本篇論文欲瞭解甲蟲蟑螂 Diploptera punctata 之咽側腺(corpora allata, CA)在胚胎發育後期所表現的活性與細胞構造的關係。利用電子 顯微鏡技術,配合細胞直徑的測量,與已知的幼蟲及成蟲期結果作比較和 討論。我們選擇第二十八天以後的胚胎 (母蟲交配後第八天視為胚胎發育 第零天),]neurosecretory granules),並且在第五十六天以後分泌量 約每隔四天為一個觀察點,至分娩前的第六十四天為止。由超顯微結構觀 察的結果發現,自第三十八天起咽側體內開始有腦神經分泌物質出現至於 其調控模式相較於已知的成蟲 D. punctata 是截然不同的;成蟲期利用 ,細胞增生並細胞變大來增加青春激素的產量並且透過同步自體吞噬消耗 大量的胞器,達到抑制細胞活性的效果。然而對胚胎期的咽側腺而言,其 細胞大小不隨活性而有明顯變化,且細胞增生也是在青春激素活胚胎期這 樣一種平滑的分泌曲線型態,再加上此時尚在發育階段,較類似於幼蟲期 ;細胞的增生或變大主要在配合蟲體的成長,與青春激素的分泌無明顯的 平行變化,可以理解其與發育完成的成蟲時期是不同的調控模式。 To realize the activity state and regulation mechanism of the corpora allata (CA) during the late embryonic stage in the cockroach, Diploptera punctata; we used electron microscopic techniques and measured cell sizes to compare the results with k From ultrastructural results, we saw neurosecretory granules from 38-day-old embryo, directly indicating the regulational signals from the brain. Simultaneously, from the observations on endoplasmic reticulum and mitochondria, we proposed two activity c About the regulation mechanism, embryos are very different from adults. Adult mated females proliferate cells and expand cell volume to produce large amount of JH, and then synchronous autophagy proceeds to digest almost all organelles to inhibit cell a Besides the smooth JH- producing pattern, there is also developmental destinations at this time, like the larval stage. Cell proliferation and expanding cell volume are not absolutely parallel with JH activity, but with growth; different from adult mate。

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