Abstract
Till date, as the technical innovations in industry, several instrument analytical methods have started in improvement of analytical efficiency and device function. However, the more accuracy we improved in the determination of metal elements result in the more complicated experimental procedures and expensive instruments in each method. What’s more, that will cost us much more manpower, time and money. Thus, the idea of on-site or even home determinations start to stand out and contribute to parts of analytical technologies developing towards miniaturization, automation and commercialization. However, when we in the analytical determination process, we still cannot eliminate the procedure to prepare the reagent and the calibration curve we need. Here, based on the idea of “miniaturized total analysis system”, this study proposed an innovative strategy for simplifying the procedure of calibration curve setting to compensate for the deficient we just mentioned above. With this novel microfluidic analytical system, we can build up a high-adaptable analytical platform in an easier way. Based on the experimental results, we have developed two analytical determination systems. For the first analytical system, we use poly(methyl methacrylate), which is cheap and easy to fabricate, as materials to build up the first-generation of concentration gradient generator Then, we couple the commercial kit with the developed microchip to approve our ability to become commercialized. For the second analytical system, we apply the idea of “microfluidic paper-based analytical devices, -PADs” to our microchip which is very popular in this decade. Only with a piece of paper, we can build up the second-generation of concentration gradient generator easily. The second-generation microchip not only solve the problems in the first generation, like complicated fabrication process and lack of injector driving force, but also couple the colorimetric method and smartphone application to determine the concentration of chromium (Ⅵ) in the groundwater.