Abstract
In the past, different kinds of analyzing or training systems have been developed for studying behavior of Drosophila melanogaster. In order to observe the different behaviors, these systems have their customized set of tools or special functions. Fruit flies have been used for decades in the study of sleep, and have become a popular model for the study of circadian behavior recently. The traditional method for monitoring fly activity involves counting the number of infrared beam crosses in individual glass tubes. However, if the fly moves away from the beam or if the fly sits right in the middle of it, too little or too much movement may be detected. Therefore, we provide a more faithful analysis system of locomotor activity and duration of sleep by using image processing and integrate with laser scanning module. We present an “Automated Real-Time Analysis System with Laser Stimulated for Sleep Behavior Study of Drosophila”. The whole system is inside a black box; the flies need to be protected against the light from outside. An artificial light-dark environment of 12:12 (h) is considered standard by led module. Real-time analyze by using image processing, we can get the fly’s position and recognize the two different flies, and rotate the two-axis motor to make laser stimulations track the specific fly immediately. In addition, we design different kind of arena and observe which one is the best for flies to live. Our version not only provide the things which the previous system can do, but also can track the more detailed information (location, speed, duration and number of sleep bout) and provide more flexibility for experiments such as sleep deprivation and courtship behavior for researchers in the future.