Abstract
Abstract Metal-responsive transcription factor 1 (MTF-1) mainly resided in the cytoplasm. Upon stimulation, it translocated into nucleus and activates gene expression. Post-translational modifications involved in different cellular processes, such as transcriptional regulation, nuclear-cytosolic transport, and protein-protein interaction. Previously, we observed that mouse MTF-1 can be modified by both SUMO-1 (small ubiquitin-like modifier 1) and SUMO-3 on lysine 627 residue. The level of sumoylation was reduced by zinc treatment in both dose- and time-dependent manners. Here we demonstrated that the level of sumoylation was also reduced by arsenic treatment. The reduced sumoylation level was depends on SUMO-interacting motif (SIM). Like SUMO-1,sumoylation sites of SUMO-3 also located in lysine 627 residue. SENP2 influenced the nuclear export of MTF-1. We also find that arsenic increased the transcription activity of K627R since the expression of downstream gene increased with the treatment. This phenomenon was different from that of zinc treatment.