Abstract
This research provides a fast scanning of organophosphorus pesticide device for diagnostic self-poisoning. They are estimated 200,000 people dead from organophosphorus pesticide poisoning around the world every year. Especially, in rural regions of the developing world, fatality rises because pesticides are more accessible and the medical management is relatively difficult. To address this unwanted need, we developed a paper-based colorimetric device which allows visual recognition of organophosphorus self-poisoning in few minutes without expensive equipments. We used filter paper printed out with wax to created a specific region for the detection of the inhibition of plasma cholinesterase activity as an important clinical bio-maker for organophosphorus and carbamates. Total operation time was shorten down to fewer than twenty minutes and portable for on-sited detection. The Visually recognizable range for plasma cholinesterase is 0.2~0.4 U / mL and it is 10 times lower than the normal plasma cholinesterase range 7~19 U / mL. Two clinical samples included during poisoned and after recovery were provided by Linkou Cheng-Kung Memorial Hospital. Organophosphorus-recovered patient sample’s the signal from is two times to that of the poisoned status assessed by using our devices. The ratios of signals obtained by using paper-based devices signal to that of commercial testing in hospital is 1.05~149 and 1.05~1.5 for poisoned samples, respectively.