Abstract
With the advance of the medical approach, the cure rate of many diseases has been improved. However, cancer still deeply affects human being. Among them, lung cancer is the one with the highest mortality and morbidity. There are so many treatments. The most effective one is surgery with about 60% of the five-year survival but it is locally restricted. Therefore, the related research still occupies a very important position. According to the latest research, the treatment combining traditional strategies and targeted therapy is considered the new hope to cure cancer. Literature indicates that when tumor grows over 2 mm in diameter, it would release the angiogenic factors, which would induce the new blood vessel growth toward the tumor due to hypoxia. Therefore, using a cell based systems to inhibit the angiogenesis of tumor had become the new strategy of cancer treatment. In medical research, in vivo test has many restrictions, such like the high cost, legal issues and ethical controversies. MEMS technology, which not only can be used to reconstruct the micro environment around tissue to reappear the function of tissue in vitro, but also predict the improved treatment effect for medication tests to reduce the cost of clinical practice. However, the past researches most use VEGF to induce angiogenesis directly. In fact, at different stage of the tumor, the angiogenesis facts released by the tumor are different. Moreover, the composition of the tumor is not only tumor cells but also many nonparenchymal cells. Thus, a chip integrating gradient generator and DEP cell patterning technology is developed in this research to reconstruct the micro environment around tumor for the studies of angiogenesis. It is expected to provide pharmaceutical information and aid in drug discovery for cancer.