Abstract
In the last few years, genome sequencing projects have produced a wealth of information on the organization of living organisms. The flood of sequence information coupled with recent advances in molecular and structure biology have led to the concept of “structural genomics”, the determination of three-dimensional protein structures on a genome-wide scale. An important use of three-dimensional structural information of proteins is to uncover clues as to a protein’s functions that are not detectable from sequence analysis. H. pylori is a micro-aerophilic Gram-negative, slow-growing, spiral-shaped and flagellated bacterium that colonizes the stomachs of an estimated half of all humans. (30% of the population in developed and up to 90% of the population in developing countries.) In a subset of humans, H. pylori infection leads to serious disease such as peptic ulcer disease, mucosa-associated lymphoid tissue lymphoma or gastric adenocarcinoma. In this paper, we will use multi-dimensional NMR techniques to determine the three-dimensional solution structures of novel protein(HP0495) In the PDB database, HP0495 has 27.397% sequence identity with a CREB binding protein. HP0495 have interacts with HP1205 which is strongly similar to translation elongation factor EF-Tu .The backbone sequential assignments were determined from six triple resonance experiments (HNCA, HNCO, HN(CO)CA, HN(CA)CO, CBCANH, and CBCA(CO)NH). Proton side chain assignments were obtained from TOCSY-HSQC, H(CC)(CO)NH, HCCH-TOCSY and HCCH-COSY. And TALOS is a database system for empirical prediction phi and psi backbone torsion angles using a combination of five kinds (HA, CA, CB, CO, N) of chemical shift assignments. Secondary structures were identified via NOE correlations, amide exchange, J coupling constants, and CSI analysis. Proton-proton distances can be estimated from the signal intensities in the 15N-NOESY-HSQC and 13C-NOESY-HSQC spectra to calculate structures. We hope to use of three-dimensional structure of HP0495 to uncover clues of it's functions。