Abstract
Glomerular play an important role of blood filtration in human body。From outside to inside are endothelia cells, glomerular basement membrane(GBM) and podocyte, respectively. These three kinds of cells act as the different size of filtration membranes to filter. For example, endothelia cells stop red blood cells crossing the blood vessel. Therefore we have to manufacture a core-shell multilayer co-axis microfibers, and culture three kinds of cell on the each layer. So that we can imitate the behavior of glomerular in human boddy. In the past research, alginate is the only material of the co-axis microfibers. Alginate will have a cross-linking reaction when we add calcium chloride solution in it. The sodium ion in alginate will be replaced by the calcium ion. The hard alginate have to hold the microfibers by its mechanical strength. The material of the inside tube is gelatin with suspended cells. We use two syringe pump to produce the outside tube and inside tube at the same time. In the first step of our experiment, we focus on the influence of podocyte after giving pressure differences. The dialysis population still high is Taiwan. The density of dialysis population ranks in top five in all over the world. And hypertension is one of the leading causes of dialysis. So we do some research on podocyte filtration after giving pressure. With the pathological behavior and results in first step, we find out some best parameters including gel inject velocity, gel species, gel concentration in second step. Cells is not easily to survive in 3D structures. In 3D structures, there are many impact factors. They will influence the survival ratio of cells. In this step, we focus on finding the best environment for cells to survive. And supply these parameters to 3D structure cells culturing. In the last step, we manufacture 3D cell tube. In this period, we observe that podocyte are sensitive to calcium ion. So we cure alginate with strontium, instead. Podocyte stretch out under the environment of strontium. It is the key factor of glomerular filtration. Besides strontium cured alginate has a more stronger mechanical strength. This is the right material for the 3D cell tube. For the future work, multi-layer 3D cell tube with endothelial cell, GBM and podocyte is our final purpose to mimic the glomerulr in vitro.