Abstract
It has recently been shown that CTX A3 internalizes into H9c2 cell (Chia-Hui Wang, phD thesis; Jin-Lang Lin, Master thesis). It is a cholesterol-sensitive process which is different from the other reported cases. In my studies, confocal microscopy and cytotoxicity assay were employed to observe the behavior of CTX A3 after modifying the concentration of cholesterol on cell plasma membrane. Dynasore, the specific inhibitor of dynamin-dependent endocytosis, was used to investigate the possible mechanism involved in the internalization of CTX A3. The results showed that the internalization of CTX A3 was cholesterol concentration- dependent and dynamin-independent process, and the cytotoxicity of CTX A3 is cholesterol-sensitive. It was suggested that the cytotoxicity of CTX A3 was correlated with the internalization of CTX A3.