Abstract
The first part of the research was exploring of guiding method, hoping to find a more efficient method to guide the moving path of fruit Flies. The second part of the research was automatic leading system in Drosophila, the purpose was to provide a automatic three-dimensional observation platform which had import and count and export functions to reduce the experimental time and implement the automation concepts. In the first part of research which used 8051 microchip to control LED matrix to produce the pattern for testing the effect of guiding. In the second part of research was to implement the automatic leading system for three-dimensional observation experiment of flies. The automatic platform can divide into four part, multi-tube exchange system, single-axis positioning system, counting and switching system, dazing and air suction system. Negative geotropism and heat intolerance was used for guiding. Counting and switching system was to control the amount of flies into biosphere. LabVIEW and DAQ was adopted for control interface for software part. Solidworks was used to construct the whole mechanism, some part of the platform was produced by 3D printer which can reduce the overall costs and implement the concept of mass production.