Abstract
Abstract Lactobacillus is a kind of probiotic bacteria, which is often applied to milk products。In this study, we tried to investigate the production of sorbitol how to produce it through metabolic engineering。Sorbitol, that possess the physiological function, has been proved to effectively reducing dental caries。If we timely add sorbitol to our daily milk-food，we could go a step further to raise its additional value。 According to Hugenholtz et. al.(2002)，within the sorbitol pathway sorbitol production is catalized by the Stl-6P-DHase gene (sorbitol-6 phosphate dehydrogenase ) and we’ll isolate the enzymatic gene from a new strain of Lactobacillus rhamnosus TCELL-1 in our laboratory, ligated with a shuttle vector pHY300PLK via electroporation into Lb. rhamnosus TCELL-1。 The result shows that the encoding Stl-6P-DHase gene is overexpressive and has been observed higher production about four fold of sorbitol than without the enzymatic gene does in Lb. rhamnosus TCELL-1。