Abstract
In 2014, pancreatic cancer is the 8th leading cause of death in cancer in Taiwan. Cancer metastasis is the major cause of death in pancreatic cancer. Five-year survival rate of pancreatic cancer is lower than 5% due to the early cancer metastasis and delayed diagnosis. In order to reduce the mortality rate of pancreatic cancer, the finding of metastatic biomarkers is essential both in pancreatic cancer prognosis and therapy. In this study, we established a pair of pancreatic ductal adenocarcinoma cells, PANC1 and its highly invasive partner PANC1-I5 as a model system to reveal the metastatic mechanism. We use two-dimensional gel electrophoresis (2D-GIGE) and matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI-TOF-MS) to exam the protein expression changes between PANC1 and PANC1-I5. Proteomic study revealed that the expression of 88 proteins are significantly modulated between PNAC1 cells and PANC1-I5 cells. Potential candidate proteins associated with pancreatic metastasis including Galectin-1 were validated by immunoblotting. Further studies have used RNA interference to evaluate the biological properties of Galectin-1 in regulating cell migration, invasion, and proliferation, in vitro. In addition, our data also demonstrated that the identified proteins such as Cathepsin D、Annexin A2, and Protein S100-A10 were potential cellular targets for modulating the metastatic process of pancreatic cancer. To sum up, proteomics approach provides numerous potential therapeutic candidates and markers for the treatment in pancreatic cancer metastasis.