Abstract
In the thesis, the methods of repairing articular cartilage is developed by a novel tissue engineering technique. Calcium phosphates contain ions of calcium and phosphate whick resemble the compostion of native bone. Thus, they are frequently used as material for scaffold on the cultivation of osteocytes. In recent studies, calcium phosphates also showed good results on the cultivation of chondrocytes.After estimation, we fabricated a dense scaffold by β-tricalcium phosphate（β-TCP）. The porosity is only 3vol%, and the pore size is almost zero. Calcium polyphosphate（CPP） can be used to manufacture a porous scaffold with three different surface phases（CPP1 is β phase with quenching，CPP2 is β phase，CPP3 is γ phase）, and the porosity is around 35~40vol%. CPP1 has the largest pore size（150um）. CPP1 and CPP2 has better mechanical properties. CPP1 has more suitable degradation time.On the cultivation of chondrocytes by different methods, we found that suspension culture can improve cell growth and cell matrix secretion, although it reduced cell adhesion slightly. As to the effect of the three different surface phases on the cultivation of chondrocytes, we found that CPP1 is the best in cell adhesion, growth,and cell matrix secretion. In conclusion, the ceramics of β phase with quenching（CPP1） is fit to be a proper tissue engineering scaffold for articular cartilage.On the cultivation of chondrocytes by complex scaffold, we found that complex scaffold can improve cell adhesion and growth, but it reduce cell matrix secretion slightly.