Abstract
To investigate the dynamic changes in the concentrations of living rat brain extracellular glucose and lactate following an acute depolarization simulation, for the first time the enzyme immobilized strategies and the three-dimensional printing (3DP) technologies are combined, and two novel enzyme-functionalized 3D-printed devices were constructed by means of (i) glutaraldehyde-facilitated crosslinking of glucose oxidase and lactate oxidase on the surfaces of 3D-printed bioreactors, and (ii) coating of peroxidase-mimicking platinum nanoparticles on the 3D-printed multi-well plates. After method’s optimization and validation, the dynamic variations of glucose and lactate concentrations in living rat brain extracellular fluids in response to the stimulus of perusing a high-K+ medium through the implanted microdialysis probe were revealed. Our analytical results and demonstrations confirm that post-printing functionalization of analytical devices manufactured using 3DP technologies can be a powerful strategy for extending the diversity and adaptability of currently existing analytical configurations.