Abstract
In our recent studies, cell surface glycosaminoglycans (GAGs) play an important role in inducing Japanese encephalitis virus (JEV) infection, the extent to which GAGs are initially involved in JEV infection and the function of binding structure are relatively unknown.Understanding the role that GAGs play in JEV infection requires establishing a specific recognition between the JEV envelope protein and structurally defined GAGs. In our recent studies, JEV is still one of the statutory contagions, which envelope protein is the entrance key of cell membrane, especially the domain III is the most interested. Using molecular biotech, we created a kind of reconstructed JEV E domain III protein based on vector pEt32a with thioredoxin which contains pEt32a、E261-420、E277-420、E292-420、E292-402 and vector pRsetA without thioredoxin which contains E261-420. We detect the kinetic constants between molecules immediately by using SPR which is biomolecular binding technology. The present studies show about the recombinant GAG-binding proteins of JEV, the binding ability is stronger in anterior predictive GAGs binding peptide E261-291 than inferior E403-420. Otherwise, pure pEt32a (thioredoxin control) also got binding ability with heparin, but we took a protein section of vector pRsetA(without thioredoxin), obtained a clear binding demonstration between JEV E261-420 and heparin. This projection is discussing between GAGs and JEV envelope protein domain III in the binding specificity, and virus entry process in 3D structure view, and proving an effective and available research direction of virus infection. Thus it is meaningful contribution in further researching of new antivirus medicine and biology.