Abstract
In this research, the objective is to analyze the residues, which are around the active site of Glycoside Hydrolases where the ligands bind, from sequence and structure. Currently, there are about 74,300 structures in Structural Classification of Proteins (SCOP)[1]. The structure of the protein can provide the information of different folds and the position where substrates bind with proteins in space. We consider the catalytic domains of the proteins, and construct the templates library which includes ligands and structures. Through mapping the relative positions, where ligands and proteins bind, to the corresponding sequences, there are some segments of the residues, and analysis of these segments. Utilized the flow chart, the analysis of the GH families is used to determine whether the protein with similar structure has the function of catalyzed glycoside or not.