Abstract
Several EP insertion lines have stress resistance was isolated in the stress screening. One of the resistant mutant, EP EP1101 was tested for lifespan, show 34% increase in the mean lifespan than control w1118. To search for the target gene nearby the EP-element insertion site in EP1101, one transcript with higher expression was detected in EP1101 flies compared to those from w1118. The target gene is CG33174 gene, which transcribes two form, long form and short form gene product. We use the different GAL4 line driver to over-express the CG33174 gene of genomic DNA or cDNA in the different tissue of fly. We found that fly have lifespan extension and have resistance to the oxidative stress. In the flybase report, the DNA sequence of CG33174 has a lipase-like domain. CG33174 gene is a novel gene, we want to analyze the gene’s function. A triacylglycerol kit was used to measure the triacylglycerol level in the fly body. The Nile red, a fluorescent dye which combines to the neutral lipid, was used in the fly section. We found that mutant fly EP1101 had decreased level in the lipid contents compared with the control w1118. On the other hand, we wondered whether the CG33174 orthologous gene in different species have the same role in the determination of lifespan extension. I made the construction of Drosophila CG33174 orthologous gene in the mouse to generate the transgenic fly. It shows that the mouse orthologous gene also extends lifespan when expressed in Drosophila. Besides, to test the fly heart performance, we found that the mutant EP1101 have the better ability to restore to normal condition than control fly.