Abstract
In recent years, biomedical materials have been widely applied in the treatment of the target cells. While tissue engineering and biomedical sensing and other new medical devices and biotechnology industries also belongs to the Government’s promotional key point. And drug delivery technology in recent years has been widely developed in the medical material field. Cell-based assays have been an important pillar of the drug discovery process to provide a simple, fast, and cost-effective tool to avoid large-scale and cost-intensive animal testing. In our study we introduces a combinatory assay platform that allows high-throughput but low-drug-dosage screening of five anti-cancer drugs as a cocktail for personalized cancer treatment. Photosensitive PEGDA hydrogel was employed for drug dosage definition through drop array formation and selective UV crosslinking process. The finally defined cocktail drugs in hydrogel will be directly released in parallel when combined with cell chips. This device is capable to combine 5 drugs with 1000 folds dynamic range in 30 second with low drug consumption for in-parallel cocktail screening process. And it is imperative to establish in vitro cell-based systems that can more realistically mimic the in vivo cell behaviors and provide more predictable results to in vivo tests. We found that cells in the 3D culture environment differ morphologically and physiologically from cells in the 2D culture environment. Drugs were highly active in 2D monolayer culture but less active and gradually lost their activity in 3D spheroids. Our cocktail chip results are analogous to a conventional method.