Abstract
A stable and sensitive modified thiocyanate method for measuring antioxidant activity has been developed. This method is used to evaluate the antioxidant activity by lipid peroxidation inhibition ability of various antioxidants. In this method, microperoxidase is used to accelerate lipid peroxidation by lipoxyl radical chain reaction thus produced, and lipid peroxide value is measured by thiocyanate method. The antioxidant activity of same common antioxidants, α-tocopherol, L-ascorbic acid, BHT and gallic acid, has been analyzed by this method. The result is comparable with the research of Hirayama’s chemiluminescence assay. However, because of time independent of nature of this equilibrium reaction, this method is especially suited for high-throughput screening.