Abstract
As polydimethylsiloxane (PDMS) is transparent, gas permeable and non-toxic, it is widely made into biochips. Living cells or bacteria present in the material and it can be successfully grown and observed. We use multi-layer soft lithography to fabricate highly integrated PDMS microfluidic chip that contains dozens of micro-flow channels, and has switch valves, pumps and other functions. With computerized program for automatic control, it operates at nanoliter level of liquid volume. We perform qualification of various functionalities such as delivery of solution, pumping and mixing in the chip. Finally, the microfluidic chips are used to do E coli culture with medium supply and lysis step for bio film control.