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A high yield robotic synthesis of 9-(4-[ 18F]-fluoro-3-hydroxymethylbutyl)guanine ([ 18F]FHBG) and 9-[(3-[ 18F]fluoro-1-hydroxy-2-propoxy)methyl]guanine([ 18F] FHPG) for gene expression imaging
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A high yield robotic synthesis of 9-(4-[ 18F]-fluoro-3-hydroxymethylbutyl)guanine ([ 18F]FHBG) and 9-[(3-[ 18F]fluoro-1-hydroxy-2-propoxy)methyl]guanine([ 18F] FHPG) for gene expression imaging

C.W. Chang, M. Lin, S.Y. Wu, C.H. Hsieh, R.S. Liu, S.J. Wang, K.L. Huang, C.H. Chen, H.E. Wang 和 Jason Chia-Hsun Hsieh
Applied radiation and isotopes, 卷.65(1), 頁碼.57-63
2007
DOI: https://doi.org/10.1016/j.apradiso.2006.06.005
PMID: 16916606
Web of Science ID: WOS:000242678800010

摘要

[ 18F]FHBG [ 18F]FHPG Gene imaging HSV1-tk Robotic system
The aim of this study was to develop an automated synthesis of 9-(4-[ 18F]-fluoro-3-hydroxymethylbutyl)guanine ([ 18F]FHBG) and 9-[(3-[ 18F]fluoro-1-hydroxy-2-propoxy)methyl]guanine ([ 18F]FHPG) using a Scanditronix Anatech RB III robotic system. [ 18F]HF was produced via 18O(p, n) 18F using a Scanditronix MC17F cyclotron. On average, a typical run produced [ 18F]FHBG and [ 18F]FHPG with an uncorrected radiochemical yield of 19% and 16%, respectively, at end of synthesis (EOS) from irradiation of 95% enriched [ 18O]water. The total synthesis time was 80 min. The retention time of [ 18F]FHBG and [ 18F]FHPG (the radio-peak) was 3.9 and 4.0 min, respectively, which was consistent with the [ 19F]FHBG and [ 19F]FHPG ultraviolet peak. The radiochemical purity was greater than 97%. A robotic, automated method for [ 18F]FHBG and [ 18F]FHPG radiosynthesis is therefore feasible. The radiation burden for the operator can be reduced as much as possible. Sufficient radioactivities of [ 18F]FHBG and [ 18F]FHPG could be obtained for non-invasive monitoring the expression of transfected gene in vivo with positron emission tomography (PET).

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