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Alternative bioreactor strategy for probing infection and production
Journal article

Alternative bioreactor strategy for probing infection and production

Yu-Chen Hu and William E. Bentley
Methods in Molecular Biology, Vol.388, pp.419-425
07/2007
PMID: 17951784

Abstract

Baculovirus infection Insect cell culture Monitoring Tubular reactor Molecular Biology Genetics
Probing the baculovirus infection process is essential in optimizing the recombinant protein production. Typically, researchers monitor the infection process in the stirred tank reactor, which, however, contains a population of cells infected at different times after virus inoculation. This chapter describes a two-stage reactor system consisting of an upstream continuous stirred tank reactor and a downstream tubular reactor with segmented plug flow for probing baculovirus infection and production. © Humana Press Inc.

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