摘要
The in vitro antimicrobial effect of cinnamaldehyde and Sporan in combination with acetic acid against Escherichia coliO157:H7 and Salmonella was investigated in Luria-Bertani broth (7 log cfu/mL) containing cinnamaldehyde or Sporan (800 and 1,000ppm) alone or in combination with 200ppm acetic acid, and incubated at 37C for up to 6h. Surviving populations of test pathogens were determined by spiral plating on selective media. E.coliO157:H7 and Salmonella were undetectable after 1h in the presence of 800ppm cinnamaldehyde. A 1,000ppm Sporan significantly reduced Salmonella and E.coliO157:H7 populations by 1.83 and 3.02 log cfu/mL within 2 and 4h, respectively. Scanning and transmission electron microscopy of oil-treated bacterial cells revealed cell structural damage and leakage of cellular content. Cinnamaldehyde was highly effective against both E.coliO157:H7 and Salmonella whereas the effect of Sporan was dependent on its concentration, exposure time and pathogen. © 2014 Wiley Periodicals, Inc.