摘要
Concerning analysis in a microfluid, so-called μTAS, the precision of the analysis is greatly affected by the flow-rate precision of the pump used. In response to this required precision of the pump, we have developed a gas-pressurized micro fluidic pump (μFPump) without any pulsation. Its applicability was examined by comparing the precision to that of a currently used pump, such as a micro-syringe pump and a diaphragm pump. The precision was compared through a model analysis, a quantitative analysis of nitrite ion in JIS K 0102 (Industry drainage examination under Japanese Industrial Standards), using a glass-made microchemical-chip as a reaction chip and a Thermal Lens Microscope as a detector. As a result of the comparison experiment, in the case of an analysis using our μFPump, a coefficient of variation (CV) of 0.7 ∼ 2.0% and a detection limit (3σ) of 0.0058 μg/ml were obtained, and it was clarified that a several-fold higher analytical precision than that of using the other pumps can be obtained. Moreover, the experiment indicates that a pump with stable flow without pulsation is necessary to carry out an accurate analysis in a microfluid. © 2005 The Japan Society for Analytical Chemistry.