Abstract
We demonstrate the feasibility of fast genetic diagnosis of coronary heart disease using a miniaturized ultrathin slab gel electrophoresis (MUSGE) device. Resolution was improved by using a discontinuous buffer system. Reproducibility was improved by taking advantage of the "capillary phenomenon" to introduce resolving gel solution, and to cast multiple numbers of gels simultaneously and reproducibly. Separation of genetic diagnosis samples was completed in 8 min, 40 times faster than by polyacrylamide slab gel electrophoresis (PAGE).