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Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans
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Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans

Wen-Han Wang, Ting-Xiu Lai, Yi-Chia Wu, Zzu-Ting Chen, Kuo-Yun TsengChung-Yu Lan
Microbiology Spectrum, 卷.10(6)
11/2022
PMID: 36416583

摘要

biofilm biofilms Candida albicans cell wall integrity pathogenesis Rap1 virulence Physiology Ecology Immunology and Microbiology (all) Genetics Microbiology (medical) Cell Biology Infectious Diseases
Rap1 (repressor activator protein 1) is a multifunctional protein, playing important roles in telomeric and nontelomeric functions in many eukaryotes. Candida albicans Rap1 has been previously shown to be involved in telomeric regulation, but its other functions are still mostly unknown. In this study, we found that the deletion of the RAP1 gene altered cell wall properties, composition, and gene expression. In addition, deletion of RAP1 affected C. albicans biofilm formation and modulated phagocytosis and cytokine release by host immune cells. Finally, the RAP1 gene deletion mutant showed attenuation of C. albicans virulence in a Galleria mellonella infection model. Therefore, these findings provide new insights into Rap1 functions that are particularly relevant to pathogenesis and virulence of C. albicans.

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https://doi.org/10.1128/spectrum.03285-22檢視
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