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Carbohydrate-encapsulated gold nanoparticles for rapid target-protein identification and binding-epitope mapping
期刊文章

Carbohydrate-encapsulated gold nanoparticles for rapid target-protein identification and binding-epitope mapping

Yu-Ju Chen, Shu-Hua Chen, Yuh-Yih Chien, Yu-Wan Chang, Hsin-Kai Liao, Chih-Yang Chang, Mi-Dan Jan, Ken-Tseng WangChun-Cheng Lin
ChemBioChem, 卷.6(7), 頁碼.1169-1173
07/2005
PMID: 15942924

摘要

Carbohydrates Glycosylation Gold Mass spectrometry Nanoparticles Proteomics Biochemistry Molecular Medicine Molecular Biology Organic Chemistry
Carbohydrate-lectin recognition plays important roles in cell-cell communication, proliferation, and differentiation. We report here a new approach of using a carbohydrate-encapsulated gold nanoparticle (shown in purple) as an affinity probe for the efficient separation and enrichment of target proteins, and then protein identification and epitope mapping by MALDI-TOF MS. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

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