摘要
Chemokine-dependent trafficking is indispensable for the effector function of antigenexperienced T cells during immune responses. In this study, we report that the water/glycerol channel aquaporin-3 (AQP3) is expressed on T cells and regulates their trafficking in cutaneous immune reactions. T cell migration toward chemokines is dependent on AQP3-mediated hydrogen peroxide (H 2 O 2 ) uptake but not the canonical water/glycerol transport. AQP3-mediated H 2 O 2 transport is essential for the activation of the Rho family GTPase Cdc4 2 and the subsequent actin dynamics. Coincidentally, AQP3-deficient mice are defective in the development of hapten-induced contact hypersensitivity, which is attributed to the impaired trafficking of antigen-primed T cells to the hapten-challenged skin. We therefore suggest that AQP3-mediated H 2 O 2 uptake is required for chemokine-dependent T cell migration in sufficient immune response. © 2012 Hara-Chikuma et al.