摘要
Background: Lysine carbamylation facilitates metal coordination for enzymatic activities. Results: Structures of dihydropyrimidinase as the apo and holoenzyme with one and two metals and its substrate/product complexes are determined. Conclusion: The structures reveal four steps in the assembly of the holoprotein with the carbamylated lysine and two metal ions. Significance: The results illustrate how proteins exploit lysines and metals to accomplish lysine carbamylation and enzymatic functions. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.