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Desktop near-field thermal-lens microscope for thermo-optical detection in microfluidics
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Desktop near-field thermal-lens microscope for thermo-optical detection in microfluidics

Adelina Smirnova, Mikhail A. Proskurnin, Kazuma MawatariTakehiko Kitamori
Electrophoresis, 卷.33(17), 頁碼.2748-2751
09/2012
PMID: 22965721

摘要

Detection in microchips Photothermal spectroscopy Thermal-lens microscopy Thermooptical detection Analytical Chemistry Biochemistry Clinical Biochemistry
A new compact near-field desktop-sized diode laser thermal-lens microscope for analysis in microfluidics was proposed. A novel beam-alignment and detection systems provided high signal stability and, along with reduced number of optical elements rendered the instrument portable. The detection of nonfluorescent model species (Fe(II)-bathophenanthroline chelate) in water showed good linearity in the range of 5 × 10 to 1 × 10 M, and the limit of detection was 3.5 × 10 M, which corresponded to 3.5 × 10 absorbance units and provided a 20-fold enhancement in sensitivity compared with existing schematic. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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