摘要
Calcium ion concentration is proposed to be involved in the regulation of the proliferate capacity of keratinocytes, based on its significant actions in the skin. These actions are mediated by Ca 2+ influx and inhibition of cell proliferation. To define Ca 2+ action in the keratinocyte we investigated its effects on the proliferation and differentiation using the primary keratinocytes model. Primary keratinocytes were incubated in DMEM (containing 1.2mM calcium ion concentration) or DK11 medium (containing 0.4 mM calcium ion concentration) or K medium (containing 0.03mM calcium ion concentration). Cell viability was assessed with the MTT assay. Crystal violet assay was evaluated the proliferation rate and colony formation size of keratinocyte. Real-time PCR used to determine the terminal differentiated keratinocyte which expressed Caspase-14. Proliferation assays and real° time PCR were correlated with either proliferation or differentiation in cultured human skin epidermal keratinocytes. High Ca 2+ concentration was inhibited the cell viability and proliferation rate of keratinocyte. Ca 2+ also increased caspases-14 expression, and inhibited cell viability, and cell colony forming efficiency. These results are consistent with Ca 2+ induction of the keratinocyte differentiation. Thus, the overall Ca 2+ actions connote protective functions for the epidermis that appear to include the triggering or acceleration of the differentiation.