摘要
An enzyme-NBD@MOF bioreactor with exemplary proteolytic performance, even after successive reuse and storage, was produced through a novel, rapid and simple multipoint immobilization technique without chemical modification of the solid support. Enzyme loading and distribution could be directly monitored from the fluorescence emission of the bioreactor. The dye molecular dimension plays a role in its overall performance. United they stand: An enzyme-NBD@MOF bioreactor (see figure) with exemplary proteolytic performance was produced by a novel, rapid and simple multipoint immobilization technique without chemical modification of the solid support. Enzyme loading and distribution can be directly monitored from the fluorescence emission of the bioreactor. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.