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Innate Immunity-Guided Macrophage-Homing Nanoplatform for Oral Tumor Immunotherapy and Real-Time Deep-Tissue Imaging in Pre-Clinical Models
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Innate Immunity-Guided Macrophage-Homing Nanoplatform for Oral Tumor Immunotherapy and Real-Time Deep-Tissue Imaging in Pre-Clinical Models

Putry Yosefa Siboro, Nhien Nguyen, Shih-Kai Lo, Fwu-Long Mi, Wen-Wei Wu, Che-Hung Wang, Yun-Ching Chen, Wei-Lun Pan, Sheng-Yao Peng, Lam-Duc-Huy Nguyen, …
Advanced materials (Weinheim), 卷.37(40), e07607
01/10/2025
PMID: 40685984

摘要

Administration, Oral Animals beta-Glucans - chemistry Carcinoma, Pancreatic Ductal - diagnostic imaging Carcinoma, Pancreatic Ductal - immunology Carcinoma, Pancreatic Ductal - therapy Cell Line, Tumor Humans Imidazoles - chemistry Immunity, Innate - drug effects Immunotherapy - methods Macrophages - immunology Macrophages - metabolism Mice Nanoparticles - chemistry Pancreatic Neoplasms - diagnostic imaging Pancreatic Neoplasms - immunology Pancreatic Neoplasms - therapy Prodrugs - chemistry Quantum Dots - chemistry Reactive Oxygen Species - metabolism Tumor Microenvironment - drug effects
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with poor prognosis and a high propensity for liver metastasis. This study presents an innate immunity-guided, macrophage (MΦ)-homing nanoplatform that enables oral delivery of theranostic agents to PDAC lesions by harnessing the migratory behavior of endogenous MΦ toward tumor-derived immune cues. The nanoplatform integrates a βGlus-R848 prodrug-constructed by conjugating β-glucans (βGlus) with the immunomodulator resiquimod (R848) via a reactive oxygen species (ROS)-responsive thioketal linker-and Ag Te quantum dots (QDs) for near-infrared II (NIR-II) imaging, forming βGlus-R848/Ag Te nanoparticles (NPs). Upon oral administration, βGlus facilitates the selective uptake of NPs by intestinal MΦ (βGlus-R848/Ag Te NPs@MΦ), which subsequently migrate to the tumor microenvironment (TME). There, elevated ROS levels trigger the release of R848, reprogramming tumor-associated MΦ from an immunosuppressive M2 to an immunoactive M1 phenotype. This immune activation remodels the stroma, enhances T cell infiltration, and transforms the TME into an immunoactive state, thereby improving therapeutic outcomes. Concurrently, Ag Te QDs enable deep-tissue NIR-II imaging for real-time visualization of PDAC progression, liver metastasis, and treatment response. Guided by innate immune signals, this MΦ-homing theranostic platform offers a promising strategy to overcome current challenges in PDAC treatment by integrating targeted immunotherapy with noninvasive, real-time disease monitoring.

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