摘要
Inorganic phosphate (Pi) uptake and translocation are crucial for plant growth and development, relying on the plasma membrane targeting of PHOSPHATE TRANSPORTER1 (PHT1) transporters. The plant‐specific endoplasmic reticulum (ER)‐resident PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 (PHF1) is structurally related to SEC12, which initiates the coat protein complex II (COPII) assembly as a guanine nucleotide exchange factor (GEF) by activating the small GTPase SAR1. In contrast, PHF1 loses the conserved catalytic residues critical for GEF activity and specifically assists the ER exit of the PHT1 transporters. However, the underlying molecular mechanism remains unknown. In this study, we showed that overexpression of Arabidopsis thaliana PHT1;1 ( At PHT1;1) in the tobacco relative Nicotiana benthamiana transient expression system caused a portion of At PHF1 to distribute into At SAR1b‐ and At SEC24a‐labelled ER exit sites (ERES). We demonstrated that At PHF1 interacts with At SAR1b and At SAR1c based on tripartite split‐GFP association in agro‐infiltrated N. benthamiana leaves and verified this interaction using miniTurbo‐based proximity labelling. We also confirmed its physiological relevance by co‐immunoprecipitating endogenous At PHF1 with At SAR1c‐GFP in Arabidopsis transgenic lines. Importantly, At PHF1 preferentially interacts with GDP‐locked At SAR1. Therefore, we propose that At PHF1 or the At PHT1;1‐ At PHF1 complex interacts with SAR1 GTPase to participate in the early step of COPII assembly for the ER export of PHT1 transporters. The plant‐specific PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 (PHF1), PHOSPHATE TRANSPORTER1 (PHT1) transporters, and SAR1 GTPase interact to participate in the early step of coat protein complex II (COPII) assembly at the ER exit sites (ERES).