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Ionization Efficiency of Dicopper Complex in Electrospray Ionization Mass Spectrometry
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Ionization Efficiency of Dicopper Complex in Electrospray Ionization Mass Spectrometry

Mei‐Kuei Lee, Yong‐Chien Ling, Pei J. HuangSung M. Wang
Journal of the Chinese Chemical Society, 卷.41(6), 頁碼.711-717
1994

摘要

Dicopper complex Electrospray ionization mass spectrometry Hemocyanin Ionization efficiency
Dicopper complex Cu 2 (PDTB)(CIO 4 ) 4 was analyzed by electrospray technique under varied conditions in order to investigate the effects of important factors on ionization efficiency. The factors taken into account are spray voltage (V n ), temperature of the drying gas, needle layer distance (D nl ), potential difference between the capillary and skimmer (V cs ), potential difference between skimmers 1 and 2 (V ss ), and concentration of analyte. The total ion current increases proportionally with V n varied from 2.0 to 4.0 kV. The ionic stales are not influenced by the variation of V n and temperature of the drying gas. Variation in nebulization efficiency caused by varied D nl appears to influence the detected ions in several ways. Structurally characteristic ions are preserved with V cs smaller than 50 V, dissociate into fragments of smaller molar mass with V cs 80 V or greater, and disappear with V cs 200 V or greater. V ss influences the relative intensities of detected ions by an effect of ion focusing. The dependence of the intensity of detected ions on analyte concentration at 10 −4 M or greater is ascribed to the principle of equilibrium in solution. Copyright © 1994 The Chemical Society Located in Taipei & Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim, Germany

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