摘要
Pancreatic ductal adenocarcinomas (PDACs) are a highly fatal malignancy, which can be identified by the elevated levels of carbohydrate antigen 125 (CA125) in human plasma. Since the effective diagnostic techniques for PDACs are inadequate, the development of a speedy and reliable sensing tool for diagnosing PDACs using CA125 detection is imperative. In this study, an ultrasensitive impedimetric immunosensor was fabricated for the electrochemical detection of CA125 in human serum. The sensing probe was fabricated by combining Er-doped graphene quantum dots (Er-GQDs) with cobalt-containing nanoporous carbon (Co@PC) (EGPC) derived from the carbonized ZIF-67, which can improve the biocompatibility and magnetic property of EGPC. After anchoring CA125 onto EGPC and coating onto the screen-printed platinum electrodes (SPPEs), the dynamic ranges are in the range of 5 × 10 −4 –500 U mL −1 and 1 × 10 −3 –500 U mL −1 with limits of detection of 3.2 × 10 −4 and 5.6 × 10 −4 U mL −1 in PBS and human serum, respectively. The SPPE||EGPC/Ab immunosensor also shows a superior recovery and stability of 97–108 % toward CA125 detection and 92.6 % after 10 times of repeated use. Moreover, the as-developed impedimetric immunosensor exhibits rapid and accurate detection of CA 125 in hospital cancer patient samples in comparison with that of the gold standard CMIA method, indicating its practical diagnostic potential. This work highlights a high-performance impedimetric immunosensor for PDAC detection using EGPC nanomaterials as a biosensing probe. The ultra-high sensitivity, selectivity, and non-invasive characteristics of magneto-controlled EGPC/Ab provide a potential platform for rapid and accurate clinical PDAC diagnosis from human serum.