Abstract
This paper reports an innovative miniaturized flow cytometer with on-line optical detection function. Microfluidic structures are integrated with buried optical waveguides on soda-lime glass substrates for on-line cell counting. A novel optical waveguide structure consists of SU-8/SOG (spin-on-glass) double-layer is demonstrated and used as an integrated optical detection device. Detection light can be guided through sample fluids and collected by the buried optical waveguides. Fluorescence labeling is not required for cell counting using the developed device. Experimental results show that diluted whole blood samples can be hydrodynamically focused and counted successfully.